The preferred total LC time is 6 h (the advantage of a longer LC time is modest). For mouse tissues, setting up material consisting of one hundred μg of protein in advance of enrichment was affordable for a single LC-MS/MS run. We also analyzed the reproducibility between multiple runs. https://andresdntyc.bloguetechno.com/where-is-glucose-converted-to-glycogen-an-overview-66924685